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Image Search Results
Journal: Oxidative Medicine and Cellular Longevity
Article Title: Anticancer Activity of Liquid Treated with Microwave Plasma-Generated Gas through Macrophage Activation
doi: 10.1155/2020/2946820
Figure Lengend Snippet: Depolarization of IL-4-pretreated Raw 264.7 macrophages at 24 h elapsed from PGNO-media treatment in various dilution ratios: (a, b) transcriptional level changes of macrophage M1 polarization-related genes, (a) including iNOS, IL6, and TNF- α , and (b) M2 polarization-related genes, including ARG, IL10, TGF- β , CCL17, EGF, and MMP9. (c) Protein level changes of iNOS. All tests were repeated three times. ( ∗ p < 0.05; † p < 0.01). (d) Flow cytometry analysis of iNOS, CD86, and CD163 proteins.
Article Snippet: LPS (L4391, Sigma) was used with a concentration of 10 ng/mL, SNAP (S-Nitroso-N-Acetyl-D,L-Penicillamine; Sigmal) was used with a concentration of 50 μ M, and cPTIO (2-(4-carboxyphenyl)-4,5-dihydro-4,4,5,5-tetramethyl-1H-imidazolyl-1-oxy-3-oxide, monopotassium salt; Cayman) was used with a concentration of 50 μ M. For staining with antibodies, anti-iNOS-PE (12-5920-80, eBioscience), anti-CD163-PE (bs-2527R-PE, Bioss), and
Techniques: Flow Cytometry